Spin-column based DNA cleanup from agarose gels
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Agarose Gel Extraction Kit is designed for high-yield recovery of DNA from agarose gel with simultaneous removal of primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities. The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify DNA in a size range between 100 bp and 10 kb. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
2 ml Collection Tubes
To be provided by you:
96-99 % Ethanol
1.5 ml microtubes
The agarose gel is dissolved in the chaotropic Extraction Buffer followed by a simple binding, washing, and eluting procedure. Before start, add 96-99 % Ethanol to the Washing Buffer as indicated on the bottle.
|Extraction Buffer||75 ml||2x 185 ml|
|Activation Buffer||6 ml||30 ml|
|Washing Buffer||add 64 ml Ethanol |
(final volume 80 ml)
|add 160 ml Ethanol to each bottle (final volume 200 ml each)|
|Elution Buffer||5 ml||25 ml|
The additional use of Isopropanol enhances yield and is recommended for fragments smaller than 200 bp or larger than 5 kbp. The optional secondary washing step minimizes the salt content of the purification product but may significantly reduce the yield of DNA fragments <200 bp.
1 Excision of the Gel:
2 Sample Preparation:
3 Column Activation:
4 Column Loading:
5 Column Washing:
Optional Secondary Washing: Recommended only for DNA >200 bp and if highly purified DNA for DNA sequencing, transfection etc. is required.
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