Pseudouridine-5'-triphosphate, Sodium Salt
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H15N2O15P3 (free acid)
Molecular Weight: 484.14 g/mol (free acid)
Exact Mass: 483.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 265 nm ±2 nm, ε 7.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Related products: HighYield T7 RNA Synthesis Kit, #RNT-101
BIOZ Product Citations:
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Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. Nucleic Acids Research. 39 (21):e142.
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Belliot et al. (2005) Norovirus proteinase-polymerase and polymerase are both active forms of RNA-dependent RNA polymerase. Journal of Virology. 79 (4):2393.
Karikó et al. (2005) Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA. Immunity. 23 (2):165.
Charette et al. (2000) Pseudouridine in RNA: what, where, how, and why. IUBMB Life. 49 (5):341.
Madore et al. (2000) Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34. Eur J Biochem. 266 (3):1128.
Goldberg et al. (1963) Comparative utilization of pseudouridine triphosphate and uridine triphosphate by ribonucleic acid polymerase. J. Biol. Chem. 238 (5):1793.