NBD-FPP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark

Shelf Life: 12 months

Molecular Formula: C₂₁H₃₀N₄O₁₀P₂

Molecular Weight: 560.43 g/mol

Purity: ≥ 95 % (HPLC)

Form: liquid (Supplied as 1 mM solution in 25 mM (NH₄)₂CO₃)

Concentration: 1 mM

Spectroscopic Properties: λ_exc 480 nm, λ_em 530 nm, ε 11.0 L mmol^-1 cm^-1 (pH 7.5)

Description:
NBD-FPP is a fluorescent analog of farnesyl pyrophosphate (FPP). It serves as lipid donor for geranylgeranyltransferases (RabGGTase, GGTase-I). NBD-FPP changes its fluorescence upon binding to protein substrates and allows for efficient fluorescence based GGTase activity assays and inhibitor screening.

BIOZ Product Citations:

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Selected References:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.