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Probes & Epigenetics

Azides of Fluorescent Dyes

(Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

Standard Dyes such as Cy3, Tamra or Texas Red have been thoroughly selected to cover the whole UV-Vis spectrum. Novel Alternative Dyes are available as well.

Preview Click Chemistry background information

Click Chemistry background information

Table 1: Available (Picolyl-) Azide-containing Fluorescent Dyes

Emission color	Dye	Azide
blue	3-Azido-7-hydroxycoumarin	3-Azido-7-hydroxycoumarin
green	BDP-FL	Azide - BDP-FL
	5-Fluorescein (5-FAM)	5-FAM-Azide
	6-Fluorescein (6-FAM)	6-FAM-Azide
	5/6-Fluorescein (5/6-FAM)	Picolyl-Azide-5/6-FAM
	AZDye 488	AZDye488-Azide AZDye488-Picolyl-Azide
	5/6-Carboxyrhodamine 110	5/6-Carboxyrhodamine 110-PEG₃-Azide
yellow	5-TAMRA	5-TAMRA-Azide
	5/6-TAMRA	5/6-TAMRA-PEG₃-Azide
	Cy3	Cy3-Azide
	Sulfo-Cy3	Sulfo-Cy3-Azide Picolyl-Azide-Sulfo-Cy3
	AZDye 546	AZDye546-Azide AZDye546-Picolyl-Azide
	AZDye 555	AZDye555-Azide AZDye555-Picolyl-Azide
orange	AZDye 594	AZDye594-Azide AZDye594-Picolyl-Azide
red	Cy5	Cy5-Azide
	Sulfo-Cy5	Sulfo-Cy5-Azide Picolyl-Azide-Sulfo-Cy5
	AZDye 647	AZDye647-Azide AZDye647-Picolyl-Azide
	Cy5.5	Cy5.5-Azide
near-IR	Cy7	Picolyl-Azide-Cy7

Products & Ordering

3-Azido-7-hydroxycoumarin CLK-FA047
Abs/Em = 404/477 nm

Azide-BDP-FL CLK-044
Abs/Em = 503/512 nm
structural analog to BODIPY® FL

5-FAM-Azide CLK-80101
Abs/Em = 494/520 nm
5-Fluorescein-Azide

6-FAM-Azide CLK-80105
Abs/Em = 496/516 nm
6-Fluorescein-Azide

Picolyl-Azide-5/6-FAM CLK-1180
Abs/Em = 490/510 nm

AZDye488-Azide CLK-1275-AZ
Abs/Em = 494/517 nm
structural analog to Alexa Fluor® 488

AZDye488-Picolyl-Azide CLK-1276-AZ
Abs/Em = 494/517 nm
structural analog to Alexa Fluor® 488

5/6-Carboxyrhodamine 110-PEG₃-Azide CLK-AZ105
Abs/Em = 501/525 nm
Azide-Fluor 488

5-TAMRA-Azide CLK-FA008
Abs/Em = 546/579 nm
5-Carboxytetramethylrhodamine-Azide

5/6-TAMRA-PEG₃-Azide CLK-AZ109
Abs/Em = 546/565 nm
5/6-Carboxytetramethylrhodamine-PEG₃-Azide, Azide-Fluor 545

Cy3-Azide CLK-046
Abs/Em = 555/570 nm

Sulfo-Cy3-Azide CLK-AZ119
Abs/Em = 553/566 nm

Picolyl-Azide-Sulfo-Cy3 CLK-1178
Abs/Em = 555/565 nm

AZDye546-Azide CLK-1283-AZ
Abs/Em = 554/570 nm
structural analog to Alexa Fluor® 546

AZDye546-Picolyl-Azide CLK-1284-AZ
Abs/Em = 554/570 nm
structural analog to Alexa Fluor® 546

AZDye555-Azide CLK-1287-AZ
Abs/Em = 555/572 nm
structural analog to Alexa Fluor® 555

AZDye555-Picolyl-Azide CLK-1288-AZ
Abs/Em = 555/572 nm
structural analog to Alexa Fluor® 555

AZDye594-Azide CLK-1295-AZ
Abs/Em = 590/617 nm
structural analog to Alexa Fluor® 594

AZDye594-Picolyl-Azide CLK-1296-AZ
Abs/Em = 590/617 nm
structural analog to Alexa Fluor® 594

Cy5-Azide CLK-047
Abs/Em = 646/662 nm

Sulfo-Cy5-Azide CLK-AZ118
Abs/Em = 647/663 nm

Picolyl-Azide-Sulfo-Cy5 CLK-1177
Abs/Em = 647/663 nm

AZDye647-Azide CLK-1299-AZ
Abs/Em = 648/671 nm
structural analog to Alexa Fluor® 647

AZDye647-Picolyl-Azide CLK-1300A-AZ
Abs/Em = 648/671 nm
structural analog to Alexa Fluor® 647

Cy5.5-Azide CLK-1059A
Abs/Em = 678/694 nm

Picolyl-Azide-Cy7 CLK-1183
Abs/Em = 753/775 nm