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Probes & Epigenetics

Azides of Fluorescent Dyes

(Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

Standard Dyes such as Cy3, Tamra or Texas Red have been thoroughly selected to cover the whole UV-Vis spectrum. Novel Alternative Dyes are available as well.

Preview Click Chemistry background information

Click Chemistry background information

Table 1: Available (Picolyl-) Azide-containing Fluorescent Dyes

Emission color	Dye	Azide
blue	3-Azido-7-hydroxycoumarin	3-Azido-7-hydroxycoumarin
green	BDP-FL (also known as BODIPY® FL)	Azide - BDP-FL
	5-Fluorescein (5-FAM)	5-FAM-Azide
	6-Fluorescein (6-FAM)	6-FAM-Azide
	5/6-Fluorescein (5/6-FAM)	Picolyl-Azide-5/6-FAM
	AF488 (also known as Alexa Fluor®488)	AF488-Azide AF488-Picolyl-Azide
	5/6-Carboxyrhodamine 110	5/6-Carboxyrhodamine 110-PEG₃-Azide
yellow	5-TAMRA	5-TAMRA-Azide
	5/6-TAMRA	5/6-TAMRA-PEG₃-Azide
	Cy3	Cy3-Azide
	Sulfo-Cy3	Sulfo-Cy3-Azide Picolyl-Azide-Sulfo-Cy3
	AF546 (also known as Alexa Fluor®546)	AF546-Azide AF546-Picolyl-Azide
	AF555 (structural analog to Alexa Fluor®555)	AF555-Azide AF555-Picolyl-Azide
orange	AF594 (also known as Alexa Fluor®594)	AF594-Azide AF594-Picolyl-Azide
red	Cy5	Cy5-Azide
	Sulfo-Cy5	Sulfo-Cy5-Azide Picolyl-Azide-Sulfo-Cy5
	AF647 (also known as Alexa Fluor®647)	AF647-Azide AF647-Picolyl-Azide
	Cy5.5	Cy5.5-Azide Picolyl-Azide-Cy5.5
near-IR	Cy7	Picolyl-Azide-Cy7

Products & Ordering

3-Azido-7-hydroxycoumarin CLK-FA047
Abs/Em = 404/477 nm

Azide-BDP-FL CLK-044
Abs/Em = 503/512 nm
also known as Azide-BODIPY® FL

5-FAM-Azide CLK-80101
Abs/Em = 494/520 nm
5-Fluorescein-Azide

6-FAM-Azide CLK-80105
Abs/Em = 496/516 nm
6-Fluorescein-Azide

Picolyl-Azide-5/6-FAM CLK-1180
Abs/Em = 490/510 nm

AF488-Azide CLK-1275
Abs/Em = 494/517 nm
also known as Alexa Fluor® 488-Azide

AF488-Picolyl-Azide CLK-1276
Abs/Em = 494/517 nm
also known as Alexa Fluor® 488-Picolyl-Azide

5/6-Carboxyrhodamine 110-PEG₃-Azide CLK-AZ105
Abs/Em = 501/525 nm
Azide-Fluor 488

5-TAMRA-Azide CLK-FA008
Abs/Em = 546/579 nm
5-Carboxytetramethylrhodamine-Azide

5/6-TAMRA-PEG₃-Azide CLK-AZ109
Abs/Em = 546/565 nm
5/6-Carboxytetramethylrhodamine-PEG₃-Azide, Azide-Fluor 545

Cy3-Azide CLK-046
Abs/Em = 555/570 nm

Sulfo-Cy3-Azide CLK-AZ119
Abs/Em = 553/566 nm

Picolyl-Azide-Sulfo-Cy3 CLK-1178
Abs/Em = 555/565 nm

AF546-Azide CLK-1283
Abs/Em = 554/570 nm
also known as Alexa Fluor® 546-Azide

AF546-Picolyl-Azide CLK-1284
Abs/Em = 554/570 nm
also known as Alexa Fluor® 546-Picolyl-Azide

AF555-Azide CLK-090
Abs/Em = 555/572 nm
structural analog to Alexa Fluor® 555

AF555-Picolyl-Azide CLK-091
Abs/Em = 555/572 nm
structural analog to Alexa Fluor® 555

AF594-Azide CLK-1295
Abs/Em = 590/617 nm
also known as Alexa Fluor® 594-Azide

AF594-Picolyl-Azide CLK-1296
Abs/Em = 590/617 nm
also known as Alexa Fluor® 594-Picolyl-Azide

Cy5-Azide CLK-047
Abs/Em = 646/662 nm

Sulfo-Cy5-Azide CLK-AZ118
Abs/Em = 647/663 nm

Picolyl-Azide-Sulfo-Cy5 CLK-1177
Abs/Em = 647/663 nm

AF647-Azide CLK-1299
Abs/Em = 648/671 nm
structural analog to Alexa Fluor® 647

AF647-Picolyl-Azide CLK-1300A
Abs/Em = 648/671 nm
structural analog to Alexa Fluor® 647

Cy5.5-Azide CLK-1059
Abs/Em = 678/694 nm

Picolyl-Azide-Cy5.5 CLK-1182
Abs/Em = 678/694 nm

Picolyl-Azide-Cy7 CLK-1183
Abs/Em = 753/775 nm