Alkyne-containing Fluorescent dyes can be used for the fluorescent labeling of Azide-tagged molecules via a Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC).
Standard Dyes (e.g. Cy3, Tamra or Texas Red) have been thoroughly selected to cover the whole UV-Vis spectrum.
| Emission color |
Dye | Product |
|---|---|---|
| green | BDP-FL | Alkyne - BDP-FL |
| 5-FAM | 5-FAM-Alkyne | |
| 6-FAM | 6-FAM-Alkyne | |
| AZDye 488 | AZDye488-Alkyne | |
| 5/6-Carboxyrhodamine 110 | 5/6-Carboxyrhodamine 110-PEG4-Alkyne | |
| yellow | 5-TAMRA | 5-TAMRA-Alkyne |
| 5/6-TAMRA | 5/6-TAMRA-PEG4-Alkyne | |
| Sulfo-Cy3 | Sulfo-Cy3-Alkyne | |
| AZDye 546 | AZDye546-Alkyne | |
| AZDye 555 | AZDye555-Alkyne | |
| orange | AZDye 594 | AZDye594-Alkyne |
| red | Sulfo-Cy5 | Sulfo-Cy5-Alkyne |
| AZDye 647 | AZDye647-Alkyne |
