2'-Fluoro-2'-deoxyadenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H15N5O12P3F (free acid)
Molecular Weight: 509.17 g/mol (free acid)
Exact Mass: 508.99 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Substrate for ATP-requiring enzymes
NMR-studies with F19
Insertion sensitive NMR reporter group in RNA
Inhibition of human DNA topoisomerases
 Stockman (2008) 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening. J. Am. Chem. Soc. 130 (18):5870.
 Scott et al. (2004) Enzymatic synthesis of 19F NMR studies of 2-fluoroadenine-substituted RNA. J. Am. Chem. Soc. 126 (38):11776.
 Liu et al. (1989) Interaction of several nucleoside triphosphate analogs and 10-hydroxycamptothecin with human DNA topoisomerases. Cancer Research 49 (6):1366.
Cho et al. (2003) Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): deciphering the basis for nucleotide selection. RNA 9 (8):970.
Rhie et al. (2003) Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion. J. Biol. Chem. 278 (41):39697.
Sun et al. (2000) Catalytic nucleic acids: from lab to applications. Pharmacol. Rev. 52 (3):325.
Jayasena (1999) Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin. Chem. 45 (9):1628.
Ono et al. (1997) 2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry. Nucleic Acids Res. 25 (22):4581.