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Biotin-11-ddUTP

Biotin-X-5-Propargylamino-ddUTP

γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2',3'-dideoxyuridine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1619-BIOX-S 25 μl (1 mM) 82,80 Add to Basket/Quote Add to Notepad
NU-1619-BIOX-L 5 x 25 μl (1 mM) 303,10 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-11-ddUTP (Biotin-X-5-Propargylamino-ddUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2',3'-dideoxyuridine-5'-triphosphate, Triethylammonium salt)
Structural formula of Biotin-11-ddUTP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C28H43N6O16P3S (free acid)

Molecular Weight: 844.66 g/mol (free acid)

Exact Mass: 844.17 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: filtered solution (30 kDa) in 10 mM Tris-HCl

Color: colorless to slightly yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
SNP-analysis[1]
DNA sequencing[2]
3'end labeling[3]

BIOZ Product Citations:

Selected References:
[1] Tong et al. (2002) Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides. Nucleic Acids Res. 30 (5):e19.
[2] Ju (2002) DNA sequencing with solid-phase-capturable dideoxynucleotides and energy transfer primers. Anal Biochem. 309 (1):35.
[3] Wang et al. (2009) Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027. BMC Microbiol. 9:14.
Edwards et al. (2001) DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry. Nucleic Acids Res. 29 (21):E104.