γ-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2'-deoxy-7-deaza-adenosine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C30H45N8O15P3S (free acid)
Molecular Weight: 882.71 g/mol (free acid)
Exact Mass: 882.19 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- PCR with Taq polymerase  & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I 
- Primer Extension with Klenow exo- [3,4]
- Reverse Transcription with MMLV Reverse Transcriptase 
Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.
Recommended Biotin-16-dATP/dATP ratio for PCR: 50% Biotin-16-dATP/ 50% dATP
Please note: The optimal final concentration of Biotin-11-dATP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Biotin-11-dATP/dATP ratio is recommended.
 Zammatteo et al. (2005) Unambiguous identification of the expressed MAGE-A genes on a DNA microarray. Clin. Chem. 51 (12):2420.
 Dauwerse et al. (1999) Two-colour FISH detection of the inv (16) in interphase nuclei of patients with acute myeloid leukaemia. Br. J. Haematol. 106 (1):111.
 Vorwerk et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N. Biotechnol. 25 (2):142.
 Beier et al. (2008) Microfluidic primer extension assay. Methods Mol Biol 822:143.
 Vankoningsloo et al. (2008) Gene expression silencing with 'specific' small interfering RNA goes beyond specificity - a study of key parameters to take into account in the onset of small interfering RNA off-target effects. FEBS J. 275 (11):2738.