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γ-[(6-Azidohexyl)-imido]-adenosine-5'-triphosphate, Sodium salt

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CLK-T12-1 1 mg 192,90 Add to Basket/Quote Add to Notepad
Structural formula of γ-[(6-Azidohexyl)-imido]-ATP (γ-[(6-Azidohexyl)-imido]-adenosine-5'-triphosphate, Sodium salt)
Structural formula of γ-[(6-Azidohexyl)-imido]-ATP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C16H28N9O12P3 (free acid)

Molecular Weight: 631.37 g/mol (free acid)

Exact Mass: 631.11 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solid

Color: white to off-white

Solubility: 10 mM Tris-HCl pH 7.5

Spectroscopic Properties: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

in vitro phosphorylation of recombinant proteins[1]

Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[(6-Azidohexyl)-imido]-ATP (compound 6[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.

The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry.

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.
For preparation of a 10 mM solution use 100 mM buffer (for example: bicarbonate, borate, phosphate and Tris) to prevent degradation at acidic pH.

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BIOZ Product Citations:

Selected References:
[1] Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.