8-Bromo-GTP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₅N₅O₁₄P₃Br (free acid)

Molecular Weight: 602.08 g/mol (free acid)

Exact Mass: 600.90 g/mol (free acid)

CAS#: 23197-98-0

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 259 nm, ε 14.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Inhibition of RNA triphosphatases^[1]
Inhibition of GTP-hydrolases^[2]
Conformational studies on syn-anti dynamics of GTP^[3]

Specific Ligands:

Yeast RNA triphosphates^[1]

Cytoskeletal protein FtsZ and tubulin^{[2, 4]}

BIOZ Product Citations:

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Selected References:
[1] Issur et al. (2009) Nucleotide analogs and molecular modeling studies reveal key interactions involved in substrate recognition by the yeast RNA triphosphatase. Nucleic Acid Res. 37:3714.
[2] Läppchen et al. (2005) GTP Analogue Inhibits Polymerization and GTPase Activity of the Bacterial Protein FtsZ without Affecting Its Eukaryotic Homologue Tubulin. Biochemistry 44 (21):7879.
[3] Hritz and Oostenbrink (2008) Hamiltonian replica exchange molecular dynamics using soft-core interactions. J. Chemical Phys. 128:144121/1.
[4] Laeppchen et al. (2008) Probing FtsZ and tubulin with C8-substituted GTP analogs reveals differences in their nucleotide binding sites. Chemistry and Biology 15:189.
Gilles Labesse et al. (2011) Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulation. Nucleic Acids Res. 39 (8):3458.