Non-hydrolyzable GTP Test Kit
Structures of non-hydrolyzable Guanosine-5'-triphosphate analogs
For research use only.
Description:
The enzymes involved in hydrolysis of nucleoside triphosphates are a major focus of biochemical research, targeting the understanding and treatment of cellular processes involved in diseases such as cancer, viral infections, cardiovascular and motional disorders. To elucidate the mechanisms of action of these enzymes, it is often desirable to trap the enzymes in their triphosphate-bound state. Since natural nucleotides are hydrolyzed at rates usually preventing such studies, nucleotide analogs are used that are hydrolyzed at much smaller rates or that are even completely resistant to hydrolysis.
The Non-hydrolyzable GTP Test Kit contains a set of 5 typical non-hydrolyzable Guanosine-5'-triphosphate analogs.
The position of the modification within the triphosphate exerts influence on its binding affinity to a particular enzyme and its hydrolysis rate.
This Kit is designed to identify the most suitable non-hydrolyzable GTP derivative for your application.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Content:
| Non-hydrolyzable Guanosine Nucleotide | Cat. No. | Amount |
|---|---|---|
| GTPαS | NU-409S | 25 μl (100 mM) |
| GpCpp | NU-405S | 100 μl (10 mM) |
| GppCp* | NU-402-5 | 5 mg |
| GppNHp* | NU-401-10 | 10 mg |
| GTPγS* | NU-412-2 | 2 mg |
* Included as a solid for stability reasons. Please dissolve in water and adjust concentration photometrically (extinction coefficient ε252nm = 13.700 M-1cm-1) prior to use to obtain a fresh solution.
Selected References:
Shiba et al. (2002) Hypocretin stimulates [ (35)]GTP gamma S binding in Hcrtr 2-transfected cell lines and in brain homogenate. Biochem. Bioph. Res. Co. 294 (3):615.
Esters et al. (2001) High-resolution crystal structure of S. cerevisiae Ypt51 (Delta C15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis. J. Mol. Biol. 298 (1):111.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta) methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.
Dye et al. (1996) Assembly of microtubules from tubulin bearing the nonhydrolyzable guanosine triphosphate analogue GMPPCP [Guanylyl 5'- (beta,gamma-methylenediphosphonate)]: Variability of growth rates and the hydrolysis of GTP. Biochemistry-US 35 (45):14331.
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Scientific citations for Jena Bioscience nk-102
5 scientific citations and 0 citation images found by Bioz.
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5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.
Molecular cell, 2026-05-08 · PMID:42105765
.. 25 mM creatine phosphate, 100 mM ATP, and 2.5 μl of RiboLock Rnase inhibitor.. Additionally, for
GTP analog treated samples Non-hydrolyzable GTP Test Kit (Jena Bioscience , Cat#NK-102 ) containing 5 analogs – GTPαS, GpCpp, GppCp, GppNHp, GTPγS – was used.. Each GTP analog was added at 0.1 mM concentration; combining all five analogs therefore .. -
Expression and purification of Escherichia coli RtcB. The rtcB gene was amplified from E. coli strain MG1655 genomic DNA by PCR using the primers: forward, 5′-TAT GCA TGC ACC ATC ATC ATC ACC ATG GTA ATT
, 2025-09-28 · DOI:
.. min, and then scanned with a Typhoon FLA 9000 imager at the FAM setting.. Studies with nonhydrolyzable GTP analogues were performed by using a
nonhydrolyzable GTP test kit (Jena Bioscience , Jena, Germany).. Studies of GTP titration reactions used half of the quantity of annealed oligonucleotides, and .. -
RNA 5ʹ terminal nucleotide determines the strength of the RIG-I/IFN signaling pathway
bioRxiv, 2023-12-22 · DOI:10.1101/2023.12.22.573000
PrePrint: .. phosphate, 100 mM ATP, and 2.5 μl of RiboProtect Hu RNase Inhibitor (Blirt #RT35).. Additionally, for
GTP analog treated samples Non-hydrolyzable GTP Test Kit (Jena Bioscience #NK-102 ) containing 5 analogs – GTPαS, GpCpp, GppCp, GppNHp, GTPγS – was used.. Each GTP analog was supplemented in 0.1 mM final concentration, which multiplied by 5 ..
