γ-[(6-Azidohexyl)-imido]-adenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C16H28N9O12P3 (free acid)
Molecular Weight: 631.37 g/mol (free acid)
Purity: ≥ 95 %
Form: white to off-white solid
Solubility: 10 mM Tris-HCl pH 7.5
Spectroscopic Properties: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
in vitro phosphorylation of recombinant proteins
Lee et al. reported a non-radioactive version of in vitro phosphorylation were γ-[(6-Azidohexyl)-imido]-ATP (compound 6) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.
The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry.
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.
For preparation of a 10 mM solution use 100 mM buffer (for example: bicarbonate, borate, phosphate and Tris) to prevent degradation at acidic pH.
 Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.