GTPγS-BDP-FL

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₄H₃₀BF₂N₈O₁₄P₃S (free acid)

Molecular Weight: 828.33 g/mol (free acid)

Exact Mass: 828.09 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: orange-brown

Concentration: 5.0 mM - 5.5 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 503 nm, λ_em 512 nm, ε 80.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

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Selected References:
Mancini et al. (2007) Exploring the Technology Landscape of 7TMR Drug Signaling Profiling. Curr. Top. Med. Chem. 15 (24):2528
Vadakkadathmeethal et al. (2007) Fluorescence-based adenylyl cyclase assay adaptable to high throughput screening. Comb. Chem. High Throughput Screen. 10 (4):289.
Korlach et al. (2004) Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs. Proc. Natl. Acad. Sci. USA 101 (9):2008.
Whelan et al. (2004) Affinity assays using fluorescence anisotropy with capillary electrophoresis separation. Anal. Chem. 76 (24):7380.
Gill et al. (2003) Low-affinity interactions of BODIPY-FL-GTPgammaS and BODIPY-FL-GppNHp with G(i)- and G(s)-proteins. Naunyn Schmiedebergs Arch. Pharmacol. 368 (3):210.
McEwen et al. (2001) Fluorescent BODIPY-GTP analogs: real-time measurement of nucleotide binding to G proteins. Anal. Biochem. 291 (1):109.