5-Ethynyl-2'-deoxycytidine (5-EdC)
For research use only.
Description:
Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2’-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine.
The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to
introduce a Biotin group (Azides of Biotin) for subsequent purification
tasks or a fluorescent group (Azides of fluorescent dyes) for subsequent
microscopic imaging [1].
Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H13N3O4
Molecular Weight: 251.24 g/mol
Exact Mass: 251.09 g/mol
CAS#: 69075-47-4
Purity: ≥ 99 % (HPLC)
Form: solid
Color: off-white
Solubility: DMSO
Spectroscopic Properties: λmax 291 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
DNA synthesis monitoring[1]
Related products:
- 5-Ethynyl-2'-deoxy-uridine (5-EdU), #CLK-N001
- Copper (II)-Sulphate (CuSO4), #CLK-MI004
- Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
- Sodium Ascorbate (Na-Ascorbate), #CLK-MI005
Selected References:
[1] Guan et al. (2011) Intracellular detection of cytosine incorporation in genomic DNA by using 5-ethynyl-2'-deoxycytidine. ChemBioChem 2 (14):2184.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
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Scientific citations for Jena Bioscience clk-n003
12 scientific citations and 0 citation images found by Bioz.
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Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state.
Journal of virology, 2026-05-04 · PMID:42080870
WT-AdV-C5 was grown in A549 cells.WT-AdV-C5 was grown in A549 cells.. EdC-labeled AdV-C5 was produced in A549 in the presence of 2.5 μM
EdC (Jena Bioscience CLK-N003 -10) added at14 h pi (48).. The non-replicating AdV-C5_dE1_GFP_dE3 vector (96, 97), which has E1/E3 deletions and expresses enhanced green .. -
Daxx mediated histone H3.3 deposition on HSV-1 DNA restricts genome decompaction and the progression of immediate-early transcription
PLoS Pathogens, 2025-08-20 · PMID:40834051
.. propagated in RPE cells and titrated in U2OS cells, as previously described [ ].. The labelling and purification of single or double labelled HSV-1 virions
with 5-Ethynyl-2’-deoxycytidine (EdC; Sigma-Aldrich, T511307) and 7-Deaza-7-ethynyl-2’-deoxyadenosine (EdA;Jena Bioscience , CLK-099) was performed as described [ , ].. Briefly, RPE cells were infected with WT or ICP0-null mutant HSV-1 at a MOI .. -
De novo assembly of RNA m 6 A modification factors into viral genome-associated nuclear bodies drives HCMV RNA accumulation
Cell Reports, 2025-07-22 · PMID:40560728
Labeling of HCMV virions for click chemistry was performed using
5-Ethynyl-2′ -deoxycytidine (EdC; Sigma-Aldrich #T511307) and 7-Deaza-7-ethynyl-2′-deoxyadenosine (EdA;Jena Bioscience #CLK-099).. Human lung fibroblast cells (IMR-90; ATCC CCL-186) were infected with unlabeled HCMV (strain AD169) ..
