Azide-containing nucleosides can be labeled with terminal Alkyne or strained Alkyne (e.g. DBCO)-tagged reporter molecules via a Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
A number of cell-permeable CLICK-functionalized nucleosides are suitable for:
DNA synthesis monitoring,
RNA synthesis monitoring,
the analysis of poly A tail dynamics or
Protein synthesis monitoring.