also known as Benzonase®
Serratia marcescens, recombinant, E. coli
For in vitro use only!
Unit Definition: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a Δ260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 1 mM DTT and 50 % [v/v] glycerol)
Concentration: 250 units/μl
Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required:
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner.
Benedik et al. (1998) Serratia marcescens and its extracellular nuclease. FEMS Microbiol. Lett. 165 (1):1.