Turbo Nuclease

For general laboratory use.

Unit Definition: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a Δ260 of 1.0 in 30 min at pH 8.0 at 37 °C.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl₂, 1 mM DTT and 50 % [v/v] glycerol)

Concentration: 250 units/μl

Applications:
Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required:

• Reduction of viscosity from cell lysates
• Prevention of cell clumping
• Elimination of unspecific protein-DNA complexes prior 2D- or native gel electrophoresis
• Removal of nucleic acids from large-scale protein preparations

Description:
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner.

• Endonuclease from Serratia macescens recombinant expressed and purified from E. coli
• Catalytic activity from pH 6 to 10 (optimal around 8.8) and temperature 0 to 44 °C
• High catalytic efficiency (34-fold greater than DNase I)
• Activity requires the presence of Mg²⁺ (optimum 2 mM)

Procedure:

• Make a fresh, cold lysis buffer in which the target protein is soluble and is compatible with downstream purification processes, e.g. minimal amount of EDTA or DTT if a Ni-NTA column will be used.
• Resuspend the thawed cell paste in lysis buffer. Use 2-10 ml Lysis Buffer for each gram of cell paste.
• Add Turbo Nuclease to 2.5 units/ml.
• A fluid 'aqueous' solution will result after 15 min.

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Selected References:
Benedik et al. (1998) Serratia marcescens and its extracellular nuclease. FEMS Microbiol. Lett. 165 (1):1.