Fluorescence based detection of DNase activity
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark, avoid freeze/thaw cycles
stable at 4 °C for up to 4 weeks
Shelf Life: 12 months
DNase Detection Kit provides a highly sensitive, fast and easy-to perform system for detection of DNase. The kit allows the detection of lowest amounts of ss- or ds-DNA degrading DNases. It is the ideal tool for contamination testing ranging from a few samples to routine process monitoring. The kit is based on a fluorescently labeled DNA probe exhibiting minimal fluorescence but showing a strong increase in fluorescence intensity in the presence of DNases. The probe is linked to the fluorophore FAM as reporter dye allowing excitation and detection with nearly all common real-time PCR cyclers or fluorescence readers.
The detection limit of the assay is
DNase I: < 1 x 10-5 units/μl
ROX reference dye
ROX Reference Dye does not take part in the detection reaction and allows therefore anormalization for non-RNase or -DNase related signal variations. We recommend to add ROX as internal standard if the instrument is compatible with the evaluation of the ROX reference signal.
Spectroscopic data of FAM (DNase Probe)
Excitation maximum: λEx = 495 nm
Emission maximum: λEm = 520 nm
Spectroscopic data of ROX (internal reference)
Excitation maximum: λEx = 576 nm
Emission maximum: λEm = 601 nm
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