For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H13N5O5
Molecular Weight: 283.24 g/mol
Exact Mass: 283.09 g/mol
Purity: ≥ 90 % (HPLC)
Form: white to off-white sticky solid
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 263 nm, ε 9.7 L mmol-1 cm-1
DNA synthesis monitoring
5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.
5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine.
The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to
 Neef et al. (2014) An azide-modified nucleoside for metabolic labeling of DNA. Chembiochem. 15 (6):789.