For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H14N2O5
Molecular Weight: 254.24 g/mol
Exact Mass: 254.09 g/mol
Purity: ≥ 98 % (HPLC)
Color: white to off-white
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 292 nm, ε 9.2 L mmol-1 cm-1 (Methanol)
DNA synthesis monitoring
5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.
5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging .
BIOZ Product Citations:
 Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.