For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C16H22N4O10
Molecular Weight: 430.37 g/mol
Purity: ≥ 90 % (LC/MS)
Form: off-white to grey amorphous solid
Solubility: DMSO, DMF, MeOH
Glycoconjugate synthesis monitoring by metabolic labeling
The tetraacetylated N-Azidoacetyl-mannosamine (Ac4ManNAz) provides a non-radioactive alternative for glycoconjugate visualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylmannosamine(ManNAc).
The resulting Azide-functionalized glycoconjugates can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.
Recommended concentration for metabolic labeling: 25-75 μM. This concentration range may serve as a starting point for an individual assay set-up.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
Spiciarich et al. (2017) Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomis. Angew. Chem. Int. Ed.129:1.