For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
Shelf Life: 12 months
Molecular Formula: C11H12N2O6
Molecular Weight: 268.22 g/mol
Purity: ≥ 99 % (HPLC)
Form: white to off-white solid
Solubility: Up to 200 mM in 1 x PBS or water by heating the obtained suspension for 1 minute by 70 °C, less soluble in DMSO
Spectroscopic Properties: λmax 286 nm; ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine.
The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
RNA synthesis monitoring
Long term storage at < -20°C. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
 Jao et al. (2008) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Nat. Acad. Sci. USA 105 (41):15779.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.