5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)

For research use only.

Description:
5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells.
5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine.

The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to

• introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
• introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes).

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₃₁H₂₈N₄O₇ (free acid)

Molecular Weight: 568.58 g/mol (free acid)

Exact Mass: 568.20 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solid

Color: colorless to slightly yellow

Solubility: DMSO (up to 200 mM tested)

Spectroscopic Properties: λ_max 289 nm, ε 12.5 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
DNA synthesis monitoring (potentially)

Related products:

• 5-Ethynyl-2'-deoxy-uridine (5-EdU), #CLK-N001
• 5-Vinyl-2'-deoxyuridine (5-VdU), #CLK-050
• 5-Azidomethyl-2'-deoxyuridine(5-AmdU), #CLK-050