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5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)

5-Dibenzylcyclooctyne-dU

Cat. No. Amount Price (EUR) Buy / Note
CLK-082-10 10 mg 385,70 Add to Basket/Quote Add to Notepad
Structural formula of 5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)
Structural formula of 5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C31H28N4O7 (free acid)

Molecular Weight: 568.58 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: colorless to slightly yellow solid

Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
DNA synthesis monitoring (potentially)

Description:
5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells.
5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine.

The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to

  • introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
  • introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes).

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