For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C31H28N4O7 (free acid)
Molecular Weight: 568.58 g/mol (free acid)
Exact Mass: 568.20 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Color: colorless to slightly yellow
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
DNA synthesis monitoring (potentially)
5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells.
5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine.
The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to
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