Azide-PEG4-aminoallyl-dUTP
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice
- to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks
- to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imaging
- to crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C23H39N6O19P3 (free acid)
Molecular Weight: 796.51 g/mol (free acid)
Exact Mass: 796.15 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ± 0.5
Spectroscopic Properties: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[1]
Selected References:
[1] Sorensen et al. (2013) Enzymatic Ligation of Large Biomolecules to DNA. ACS Nano 7 (9):8098.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1705S | 50 μl (10 mM) | 157,60 € |
| NU-1705L | 5 x 50 μl (10 mM) | 461,30 € |
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Scientific citations for Jena Bioscience nu-1705
4 scientific citations and 0 citation images found by Bioz.
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Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
, 2025-01-28 · DOI:
.. of the “carrier” of the present disclosure for monoclonal clustering.. FIG. 3 is non-limiting exemplary gel graph showing the attempted incorporation of
Azide-PEG4-aminoallyl-dUTP (Jena Biosciences ; #NU-1705S ), referred to in this example as azide-PEG4-dUTP or Az-U, onto a 5-Carboxyfluorescein (5′ FAM) labelled oligonucleotide primer by TdT3-2 or a commercial NEB TdT.. As indicated above individual lanes, different reactions were set up .. -
DNA-enhanced CuAAC ligand enables live-cell detection of intracellular biomolecules
bioRxiv, 2022-11-10 · DOI:10.1101/2022.11.10.515969
PrePrint: .. and reconstituted to 100 μM with nuclease-free H 2 O.. We mixed
250 μl of 1mM ofAzide-PEG4-aminoallyl-dUTP (Jena Bioscience ), 100 μl of 10X transferase buffer, 100 μl of 100μM 15mer ssDNA oligo, 30 μl of TdT enzyme (20,000U/ml), 100 μl of 10X CoCl 2 , 420 μl of NF-H 2 O in a total reaction volume of 1000 μl.. The reaction was vortexed for several seconds then incubated at .. -
Super-resolution sequencing
, 2021-07-20 · DOI:
We obtained a custom synthesized oligonucleotide (Genelink) containing a partner 2 sequence containing a 5′ hexynyl linker, a disulphide, a 3′ phosphate (5′ [5Hexynyl][SS-C5]TTATACATCTA (SEQ ID NO: 1) [Phos] 3′) and clicked it to an azide containing nucleotide,
Azide-PEG4-aminoallyl-dUTP (Jena Biosciences ) using the Oligo-Click Kit according to kit protocol (BaseClick, Germany) to make an oligo-tagged nucleotide.
