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NBD-FPP

{3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino),
-dodeca-2,6,10-trien-1} pyrophosphate

Fluorescent lipid donor for protein geranylgeranyltransferases (RabGGTase, GGTase-I)

Cat. No. Amount Price (EUR) Buy / Note
LI-013 20 μl 180,00 Add to Basket/Quote Add to Notepad

Structural formula of NBD-FPP

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark

Shelf Life: 12 months

Molecular Formula: C21H30N4O10P2

Molecular Weight: 560.43 g/mol

Purity: ≥ 95 % (HPLC)

Form: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)

Concentration: 1 mM

Spectroscopic Properties: λexc 480 nm; λem 530 nm; ε 11.0 L mmol-1 cm-1 (pH 7.5)

Description:
NBD-FPP is a fluorescent analog of farnesyl pyrophosphate (FPP). It serves as lipid donor for geranylgeranyltransferases (RabGGTase, GGTase-I). NBD-FPP changes its fluorescence upon binding to protein substrates and allows for efficient fluorescence based GGTase activity assays and inhibitor screening.

Selected References:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.