Fluorescent lipid donor for protein geranylgeranyltransferases (RabGGTase, GGTase-I)
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark
Shelf Life: 12 months
Molecular Formula: C21H30N4O10P2
Molecular Weight: 560.43 g/mol
Purity: ≥ 95 % (HPLC)
Form: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)
Concentration: 1 mM
Spectroscopic Properties: λexc 480 nm; λem 530 nm; ε 11.0 L mmol-1 cm-1 (pH 7.5)
NBD-FPP is a fluorescent analog of farnesyl pyrophosphate (FPP). It serves as lipid donor for geranylgeranyltransferases (RabGGTase, GGTase-I). NBD-FPP changes its fluorescence upon binding to protein substrates and allows for efficient fluorescence based GGTase activity assays and inhibitor screening.
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.