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5-TCO-PEG4-dUTP

5-trans-Cyclooctene-PEG4-dUTP

Cat. No. Amount Price (EUR) Buy / Note
CLK-035S 200 μl (1 mM) 130,84 Add to Basket/Quote Add to Notepad
CLK-035L 5 x 200 μl (1 mM) 523,35 Add to Basket/Quote Add to Notepad
Structural formula of 5-TCO-PEG4-dUTP (5-trans-Cyclooctene-PEG4-dUTP)
Structural formula of 5-TCO-PEG4-dUTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C32H53N4O21P3 (free acid)

Molecular Weight: 922.70 g/mol (free acid)

Exact Mass: 922.24 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 240 nm, ε 10.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by

  • PCR with Taq polymerase in-house data

Description:
5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice

  • to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging
  • to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins