5-TCO-PEG₄-dUTP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₃₂H₅₃N₄O₂₁P₃ (free acid)

Molecular Weight: 922.70 g/mol (free acid)

Exact Mass: 922.24 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 240 nm, ε 10.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by

• PCR with Taq polymerase ^{in-house data}

Description:
5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice

• to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks
• to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging
• to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins

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