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Aminoallyl-dUTP-5/6-TAMRA

Tetramethyl-Rhodamine-5-dUTP

5-(3-Aminoallyl)-2'-deoxyuridine-5'triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-TAM 30 μl (1 mM) 214,79 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-5/6-TAMRA (Tetramethyl-Rhodamine-5-dUTP, 5-(3-Aminoallyl)-2'-deoxyuridine-5'triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-5/6-TAMRA
excitation and emission spectrum of 5/6-TAMRA
excitation and emission spectrum of 5/6-TAMRA

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C37H40N5O18P3 (free acid)

Molecular Weight: 935.66 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile clear aqueous solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λabs 545 nm, λem 575 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I in-house data, [1,2]

Description:
Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/ 65% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Selected References:
[1] Idziak et al. (2014) Insight into the Karyotype Evolution of Brachypodium Species Using Comparative Chromosome Barcoding. PLOS One 9(3):e93503.
[2] Hasterok et al. (2006) Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization. Genetics 173:349.