Aminoallyl-dUTP-5/6-TAMRA

excitation and emission spectrum of 5/6-TAMRA

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₃₇H₄₀N₅O₁₈P₃ (free acid)

Molecular Weight: 935.66 g/mol (free acid)

Exact Mass: 935.16 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: filtered solution (30 kDa) in 10 mM Tris-HCl

Color: pink to red

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_abs 545 nm, λ_em 575 nm, ε 90.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I ^{in-house data, [1,2]}

Description:
Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/ 65% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

BIOZ Product Citations:

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Selected References:
[1] Idziak et al. (2014) Insight into the Karyotype Evolution of Brachypodium Species Using Comparative Chromosome Barcoding. PLOS One 9(3):e93503.
[2] Hasterok et al. (2006) Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization. Genetics 173:349.