2'-Deoxyadenosine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers)
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-426S | 100 μl (10 mM) | 165,50 | Add to Basket/Quote Add to Notepad |
| NU-426L | 5 x 100 μl (10 mM) | 484,70 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O11P3S (free acid)
Molecular Weight: 507.24 g/mol (free acid)
Exact Mass: 506.98 g/mol (free acid)
CAS#: 64145-28-4
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
BIOZ Product Citations:
Selected References:
Gaur et al. (1993) Enzymatic RNA synthesis with deoxynucleoside 5'-O- (1-thiotriphosphates). FEBS Lett. 315 (1):56.
Abbotts et al. (1988) Studies on the mechanism of Escherichia coli DNA polymerase I large fragment. Effect of template sequence and substrate variation on termination of synthesis. J. Biol. Chem. 263 (29):15094.
Nakamaye et al. (1988) Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. Nucleic Acids Res. 16 (21):9947.
Nyren et al. (1997) Detection of single-base changes using a bioluminometric primer extension assay. Anal. Biochem. 244 (2):367.
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