Guanosine-5'-(γ-thio)-triphosphate, Tetralithium salt
For research use only!
Shipping: shipped on dry ice
Storage Conditions: store at -20 °C
Shelf Life: 6 months after date of delivery
Molecular Formula: C10H16N5O13P3S (free acid)
Molecular Weight: 539.25 g/mol (free acid)
Exact Mass: 538.97 g/mol (free acid)
Purity: ≥ 90 % (HPLC),
contains < 10 % GDP (HPLC)
Color: white to off-white
Spectroscopic Properties: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Functional assay for GPCRs[1, 2]
Heterotrimeric and monomeric G-proteins
for P2Y-like receptor and for P2Y13 receptor
Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Cascio et al. (2010) In vitro and in vivo pharmacological characterization of two novel selective cannabinoid CB (2) receptor inverse agonists. Pharmacological research 61:349.
 Obara et al. (2010) Agonist-dependent attenuation of micro-opioid receptor-mediated G-protein activation in the dorsal root ganglia of neuropathic rats. J. Neural Transmission 117:421.
 Cascio et al. (2010) Evidence that the plant cannabinoid cannabigerol is a highly potent alpha2-adrenoreceptor agonist and moderately potent SHT1A receptor antagonist. Brit. J. Pharmacol. 159:129.
 Koval et al. (2010) Europium-labeled GTP as a general nonradioactive substitute for [35S]GTPyS in high-throughput G-protein studies. Analytical Biochem. 397:202.
 Yamazaki et al. (2010) Mechanism for the regulation of mammalian cGMP phosphodiesterase 6. 2: Isolation and charachterization of the transducin-activated form. Mol. Cell. Biochem. 339:235.
 Daniele et al. (2011) Agonist-induced desensitization/resensitization of human G protein- coupled receptor 17: a functional cross-talk between purinergic and cysteinyl-leukotriene ligands. J. Pharmacol. Exp. Ther. 338 (2):559.
 Marteau et al. (2003) Pharmacological characterization of human P2Y13 receptor. Mol. Pharmacol. 64 (1):104.
Schmohl et al. (2012) Functional analysis of Rho GTPase activation and inhibition in a bead-based miniaturized format.Methods. Mol. Biol. 827: 271.
Martin-Galiano AJ et al. (2011) Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor. J. Bio. Chem. 286 (22):19789.
Abenza et al. (2010) Aspergillus RabB Rab5 integrates acquisition of degradative identity with the long distance movement of early endosomes. Mol. Biol. Cell. 21 (15):2756.
Beuve et al. (2005) Nitric Oxide-dependent allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Kirilenko et al. (2002) GTP-induced membrane binding and ion channel activity of annexin VI: Is annexin VI a GTP biosensor? Biophys. J. 82 (5):2737.
Shiba et al. (2002) Hypocretin stimulates [ (35)]GTP gamma S binding in Hcrtr 2-transfected cell lines and in brain homogenate. Biochem. Biophys. Res. Commun. 294 (3):615.
Aiyar et al. (1994) Human At (1) receptor is a single-copy gene - characterization in a stable cell-line. Mol. Cell. Biochem. 131 (1):75.
Ashktorab et al. (1994) Presence of GTP-binding proteins in the plasma-membrane of the Phycomyces sporangiophore. Exp. Mycol. 18 (2):139.
Schwemmle et al. (1994) The interferon-induced 67-kDa Guanylate-binding protein (HGbp1) is a GTPase that converts GTP to GMP. J. Biol. Chem. 269 (15):11299.
Noel et al. (1993) The 2.2 Å crystal structure of transducin- alpha complexed with GTP gamma S. Nature 366:654.