ATP-γ-AmNS

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₀H₂₃N₆O₁₅P₃S (free acid)

Molecular Weight: 712.41 g/mol (free acid)

Exact Mass: 712.02 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 5.0 mM - 5.5 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 323 nm, ε 4.2 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), λ_exc 323 nm, λ_em 461 nm

Applications:
Quenching of protein fluorescence^[1]
Identification of binding sites in E-coli isocitrate dehydrogenase kinase^[1]
Estimation of ADP-binding sites in membrane bound adenine-nucleotide carrier^[2]
Substrate for snake venom phosphodiesterase^[3]
Intramolecular energy transfer^[4]
E-coliIsocitrate dehydrogenase kinase/phosphatase^[1]

BIOZ Product Citations:

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Selected References:
[1] Rittinger et al. (1996) Escherichia coli isocitrate dehydrogenase kinase/phosphatase. Overproduction and kinetics of interaction with its substrates by using intrinsic fluorescence and fluorescent nucleotide analogues. Eur. J. Biochem. 237 (1):247.
[2] Block et al. (1984) Substrate-site interactions in the membrane-bound adenine-nucleotide carrier as disclosed by ADP and ATP analogs. Biochim Biophys Acta. 767 (2):369.
[3] Pollack et al. (1982) Fluorescent nucleotide triphosphate substrates for snake venom phosphodiesterase. Anal. Biochem. 127 (1):81.
[4] Yarbrough et al. (1980) Stacking interactions in fluorescent nucleotide analogs containing 1-aminonaphthalene-5-sulfonate at the phosphoryl terminus. J. Biol. Chem. 255 (20):9907.