EDA-GTP-BDP-FL

For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₇H₃₅BF₂N₉O₁₆P₃ (free acid)

Molecular Weight: 883.35 g/mol (free acid)

Exact Mass: 883.15 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: orange

Concentration: 5.0 mM - 5.5 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 503 nm, λ_em 512 nm, ε 80.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Selected References:
Surviladze et al. (2010) Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay. J. Biomol. Screen 15 (1):10.
Jameson et al. (2007) Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity. Anal. Chem 79 (3):1158.
Bae et al. (2006) Automated single-cell electroporation. Biotechniques 41 (4):399.
Jameson et al. (2005) IReal-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues. J. Biol. Chem. 280 (9):7712.
Willard et al. (2005) A direct fluorescence-based assay for RGS domain GTPase accelerating activity. Anal. Biochem. 340 (2):341.