7-Propargylamino-7-deaza-dATP-Cy5

excitation and emission spectrum of Cy5

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₄₇H₅₈N₇O₁₉P₃S₂ (free acid)

Molecular Weight: 1182.05 g/mol (free acid)

Exact Mass: 1181.24 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: blue

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 649 nm, λ_em 670 nm, ε 250.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation by primer extension^[1]
Incorporation by PCR^[2]

Selected References:
[1] Braslavsky et al. (2003) Sequence information can be obtained from single DNA molecules. Proc. Natl. Acad. Sci. USA. 100 (7):3960.
[2] Tasara et al. (2003) Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA. Nucleic Acids Res. 31 (10):2636.