(ApppG), GP3A, GpppA, G(5')ppp(5')A
P1-(5'-Adenosyl) P3-(5'-guanosyl) triphosphate, Sodium salt
Cat. No. | Amount | Price (EUR) | Buy / Note |
---|---|---|---|
NU-941S | 10 μl (100 mM) | 75,10 | Add to Basket/Quote Add to Notepad |
NU-941L | 5 x 10 μl (100 mM) | 337,30 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C20H27N10O17P3 (free acid)
Molecular Weight: 772.41 g/mol (free acid)
Exact Mass: 772.08 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λ 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Synthesis of mRNA with a non-functional cap analog (ApppG) to estimate the level of cap-independent translation.[1]
Investigation of stress related (Near UV and oxidation) product formation in bacteria[2-4]
BIOZ Product Citations:
Selected References:
[1] Nowakowska et al. (2014) Cap analogs containing 6-thioguanosine--reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules. Org. Biomol. Chem. 12 (27):4841.
[2] Kramer et al. (1988) Near-UV stress in Salmonella typhimurium: 4-thiouridine in tRNA, ppGpp, and ApppGpp as components of an adaptive response. J. Bacteriol. 170 (5):2344.
[3] Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
[4] VanBogelen et al. (1987) Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. J. Bacteriol. 169 (1):26.