8-Azido-adenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H15N8O13P3 (free acid)
Molecular Weight: 548.19 g/mol (free acid)
Exact Mass: 548.00 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 281 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP)
Identification of protein ATP-binding sites
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Chen et al. (2004) Chain Termination and Inhibition of Saccharomyces cerevisiae Poly (A) Polymerase by C-8-modified ATP analogs. J. Biol. Chem. 279 (39):40405.
 Olcott et al. (1998) Localization and Characterization of Two Nucleotide-binding Sites on the Anaerobic Ribonucleotide Reductase from Bacteriophage T4. J. Biol. Chem. 273 (38):24853.