Vinyl-containing nucleosides can be labeled with Tetrazine-tagged reporter molecules via Copper-free Tetrazine-Alkene Ligation that allows to introduce a Biotin group for subsequent purification (via Tetrazine-containing Biotinylation Reagents → Click Chemistry) or a fluorescent group for subsequent microscopic imaging (via Tetrazine-containing Fluorescent Dyes → Click Chemistry).
They are suitable for DNA synthesis monitoring → Click Chemistry or RNA synthesis monitoring → Click Chemistry, respectively.
