2-Hydroxy-2'-deoxyadenosine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O13P3
Molecular Weight: 507.18 g/mol
Exact Mass: 507.00 g/mol
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 292 nm, ε 10.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
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Xia et al. (2005) Transient state kinetic studies of the MutT-catalyzed nucleoside triphosphate pyrophosphohydrolase reaction. Biochemistry 44 (46):15334.
Kamiya et al. (2004) Mutagenesis by damaged deoxyribonucleotides and its prevention by MutT-type hydrolyzing enzymes. Nucleic Acids Symp Ser (Oxf) 48:271.
Satou et al. (2003) Mutations induced by 2-hydroxy-dATP during in vitro replication with a HeLa extract. Nucleic Acids Res Suppl. 3:325.
Satou et al. (2003) Mutagenic effects of 2-hydroxy-dATP on replication in a HeLa extract: induction of substitution and deletion mutations. NNucleic Acids Res. 31 (10):2570.
Saraswat et al. (2002) Interactions of the products, 8-oxo-dGMP, dGMP, and pyrophosphate with the MutT nucleoside triphosphate pyrophosphohydrolase. Biochemistry 41 (52):15566.
Kamiya et al. (2001) Hydrolysis of oxidized nucleotides by the Escherichia coli Orf135 protein. Biochem Biophys Res Commun. 48:271.
Kamiya et al. (2000) Two DNA polymerases of Escherichia coli display distinct misinsertion specificities for 2-hydroxy-dATP during DNA synthesis. Biochemistry 39 (31):9508.
Kamiya et al. (2000) 2-Hydroxy-dATP is incorporated opposite G by Escherichia coli DNA polymerase III resulting in high mutagenicity. Nucleic Acids Res. 28 (7):1640.