8-Oxo-2'-deoxyguanosine-5'-triphosphate, Sodium salt
8-Hydroxy-2'-deoxyguanosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O14P3 (free acid)
Molecular Weight: 523.18 g/mol (free acid)
Exact Mass: 522.99 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 245 nm, ε 12.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Influence on base excision repair
Influence on erroneous incorporation by DNA-polymerases
Hydrolysis to 8-oxo-dGMP by E.-coli MutT
Triggering cell senescence through formation of ROS
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Suzuki et al. (2010) Effect of base excision repair proteins on mutagenesis by 8-oxo-7, 8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. DNA Repair 9:542.
 Katafuchi et al. (2010) Critical amino acids in human DNA polymerases η and κ involved in erroneous incorporation of oxidized nucleotides. Nucleic Acids Res. 38:859.
 Nakamura et al. (2010) Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base. J. Biol. Chem. 285:444.
 Raia et al. (2009) Continous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. PNAS USA 106:169.
Yoshida H. et al. (2011) Increase in CpG DNA-induced inflammatory responses by DNA oxidation in macrophages and mice. Free Radic Biol Med. 51 (2):424.
Petrie et al. (2010) Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs. Nucleic Acids Res. 38 (22):8095.
Ramírez (2004) Gene of Bacillus subtilis Encodes a Functional Antimutator 8-Oxo- (dGTP/GTP)ase and Is under Dual Control of Sigma A and Sigma F RNA Polymerases. Journal of Bacteriology 186 (4):1050.
Zaccolo et al. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. Journal of Molecular Biology 285 (2):775.
Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589.
Kai et al. (2002) An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates. Nucl. Acids Res. 30 (2):569.
Canitrot et al. (2000) Nucleotide excision repair DNA synthesis by excess DNA polymerase beta: a potential source of genetic instability in cancer cells. FASEB J. 14 (12):1765.
Nampalli et al. (2000) Efficient synthesis of 8-oxo-dGTP: A mutagenic nucleotide. Bioorg. Med. Chem. Lett. 10 (15):1677.
Loeb et al. (1999) Lethal mutagenesis of HIV with mutagenic nucleoside analogs. Proc. Natl. Acad. Sci. USA 96:1492.