Vitrification Starter Kit
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C / ambient temperature
Shelf Life: 12 months
Optimization of the vitrification process in cryo-EM sample preparation
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.
|Surfactants and Cryoprotectants||Amount||Conc.||CMC||Class|
|Fluorinated Octyl Maltoside (FOM)||100 μl||0.41% (w/v)||0.07% (w/v)||non-ionic detergent|
|Hexadecyl-trimethyl-ammonium Bromide (CTAB)||100 μl||0.34% (w/v)||0.03% (w/v)||cationic detergent|
|n-Decyl-ß-D-Maltoside (DM)||100 μl||0.87% (w/v)||0.09% (w/v)||non-ionic detergent|
|n-Decyl-α-D-Maltoside (DαM)||100 μl||0.46% (w/v)||0.08% (w/v)||non-ionic detergent|
|n-Dodecyl-ß-D-Maltoside (DDM)||100 μl||0.09% (w/v)||0.01% (w/v)||non-ionic detergent|
|Sodium Deoxycholate||100 μl||1.66% (w/v)||0.17% (w/v)||anionic detergent|
|Triton X-100||100 μl||0.15% (w/v)||0.01% (w/v)||non-ionic detergent|
|Tween 20||100 μl||1% (w/v)||0.01% (w/v)||non-ionic detergent|
|CHAPSO||100 μl||2.5% (w/v)||0.5% (w/v)||zwitterionic detergent|
|Amphipol A8-35||100 μl||5% (w/v)||anionic surfactant|
|Glycerol||1 ml||30% (w/v)||cryoprotectant|
Quantifoil Holey Carbon Films
20x Quantifoil™ R 1.2/1.3 on 200 copper mesh
20x Quantifoil™ R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh
20x Quantifoil™ R 2/1 on 300 copper mesh
20x Quantifoil™ R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh
 Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
 Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
 Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
 Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
 Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
 Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.