Vitrification Starter Kit
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| X-CEM-301 | 1 Kit | 1.198,20 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C / ambient temperature
Shelf Life: 12 months
Applications:
Optimization of the vitrification process in cryo-EM sample preparation
Description:
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.
Content:
| Surfactants and Cryoprotectants | Amount | Conc. | CMC | Class |
| Fluorinated Octyl Maltoside (FOM) | 100 μl | 0.41% (w/v) | 0.07% (w/v) | non-ionic detergent |
| Hexadecyl-trimethyl-ammonium Bromide (CTAB) | 100 μl | 0.34% (w/v) | 0.03% (w/v) | cationic detergent |
| n-Decyl-ß-D-Maltoside (DM) | 100 μl | 0.87% (w/v) | 0.09% (w/v) | non-ionic detergent |
| n-Decyl-α-D-Maltoside (DαM) | 100 μl | 0.46% (w/v) | 0.08% (w/v) | non-ionic detergent |
| n-Dodecyl-ß-D-Maltoside (DDM) | 100 μl | 0.09% (w/v) | 0.01% (w/v) | non-ionic detergent |
| Sodium Deoxycholate | 100 μl | 1.66% (w/v) | 0.17% (w/v) | anionic detergent |
| Triton X-100 | 100 μl | 0.15% (w/v) | 0.01% (w/v) | non-ionic detergent |
| Tween 20 | 100 μl | 1% (w/v) | 0.01% (w/v) | non-ionic detergent |
| CHAPSO | 100 μl | 2.5% (w/v) | 0.5% (w/v) | zwitterionic detergent |
| Amphipol A8-35 | 100 μl | 5% (w/v) | anionic surfactant | |
| Glycerol | 1 ml | 30% (w/v) | cryoprotectant |
Quantifoil Holey Carbon Films
20x Quantifoil™ R 1.2/1.3 on 200 copper mesh
20x Quantifoil™ R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh
20x Quantifoil™ R 2/1 on 300 copper mesh
20x Quantifoil™ R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh
BIOZ Product Citations:
Selected References:
[1] Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
[2] Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
[3] Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
[4] Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
[5] Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
[6] Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.
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