Vitrification Starter Kit
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C / ambient temperature
Shelf Life: 12 months
Optimization of the vitrification process in cryo-EM sample preparation
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.
|Surfactants and Cryoprotectants||Amount||Conc.||CMC||Class|
|Fluorinated Octyl Maltoside (FOM)||100 μl||0.41% (w/v)||0.07% (w/v)||non-ionic detergent|
|Hexadecyl-trimethyl-ammonium Bromide (CTAB)||100 μl||0.34% (w/v)||0.03% (w/v)||cationic detergent|
|n-Decyl-ß-D-Maltoside (DM)||100 μl||0.87% (w/v)||0.09% (w/v)||non-ionic detergent|
|n-Decyl-α-D-Maltoside (DαM)||100 μl||0.46% (w/v)||0.08% (w/v)||non-ionic detergent|
|n-Dodecyl-ß-D-Maltoside (DDM)||100 μl||0.09% (w/v)||0.01% (w/v)||non-ionic detergent|
|Sodium Deoxycholate||100 μl||1.66% (w/v)||0.17% (w/v)||anionic detergent|
|Triton X-100||100 μl||0.15% (w/v)||0.01% (w/v)||non-ionic detergent|
|Tween 20||100 μl||1% (w/v)||0.01% (w/v)||non-ionic detergent|
|CHAPSO||100 μl||2.5% (w/v)||0.5% (w/v)||zwitterionic detergent|
|Amphipol A8-35||100 μl||5% (w/v)||anionic surfactant|
|Glycerol||1 ml||30% (w/v)||cryoprotectant|
Quantifoil Holey Carbon Films
20x Quantifoil™ R 1.2/1.3 on 200 copper mesh
20x Quantifoil™ R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh
20x Quantifoil™ R 2/1 on 300 copper mesh
20x Quantifoil™ R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
 Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
 Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
 Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
 Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
 Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.