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Cryo-EM V-Kit

Vitrification Starter Kit

Cat. No. Amount Price (EUR) Buy / Note
X-CEM-301 1 Kit 937,00 Add to Basket/Quote Add to Notepad

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C / ambient temperature

Shelf Life: 12 months

Applications:
Optimization of the vitrification process in cryo-EM sample preparation

Description:
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.

Content:

Surfactants and Cryoprotectants Amount Conc. CMC Class
Fluorinated Octyl Maltoside (FOM) 100 μl 0.41% (w/v) 0.07% (w/v) non-ionic detergent
Hexadecyl-trimethyl-ammonium Bromide (CTAB) 100 μl 0.34% (w/v) 0.03% (w/v) cationic detergent
n-Decyl-ß-D-Maltoside (DM) 100 μl 0.87% (w/v) 0.09% (w/v) non-ionic detergent
n-Decyl-α-D-Maltoside (DαM) 100 μl 0.46% (w/v) 0.08% (w/v) non-ionic detergent
n-Dodecyl-ß-D-Maltoside (DDM) 100 μl 0.09% (w/v) 0.01% (w/v) non-ionic detergent
Sodium Deoxycholate 100 μl 1.66% (w/v) 0.17% (w/v) anionic detergent
Triton X-100 100 μl 0.15% (w/v) 0.01% (w/v) non-ionic detergent
Tween 20 100 μl 1% (w/v) 0.01% (w/v) non-ionic detergent
CHAPSO 100 μl 2.5% (w/v) 0.5% (w/v) zwitterionic detergent
Amphipol A8-35 100 μl 5% (w/v) anionic surfactant
Glycerol 1 ml 30% (w/v) cryoprotectant

Quantifoil Holey Carbon Films
20x Quantifoil™ R 1.2/1.3 on 200 copper mesh
20x Quantifoil™ R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh
20x Quantifoil™ R 2/1 on 300 copper mesh
20x Quantifoil™ R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh

Selected References:
[1] Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
[2] Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
[3] Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
[4] Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
[5] Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
[6] Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.