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5-Vinyl-uridine (5-VU)

5-Ethenyl-uridine

Cat. No. Amount Price (EUR) Buy / Note
CLK-049-10 10 mg 114,75 Add to Basket/Quote Add to Notepad
CLK-049-50 50 mg 395,55 Add to Basket/Quote Add to Notepad
Structural formula of 5-Vinyl-uridine (5-VU) (5-Ethenyl-uridine)
Structural formula of 5-Vinyl-uridine (5-VU)

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C11H14N2O6

Molecular Weight: 270.24 g/mol

CAS#: 55520-64-4

Purity: ≥ 98 % (HPLC)

Form: white to off-white powder

Solubility: DMSO, methanol

Spectroscopic Properties: λmax 288 nm, ε 8.5 L mmol-1 cm-1 (Methanol)

Applications:
RNA synthesis monitoring (potentially)

Description:
5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring[1].

The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

Related products: 5-Ethynyl-uridine (5-EU), #CLK-N002

Selected References:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.