5-Vinyl-uridine (5-VU)

For general laboratory use.

Description:
5-VU (5-Vinyl-uridine) can be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells ^[1]. 5-VU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine.

The resulting vinyl-functionalized RNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₄N₂O₆

Molecular Weight: 270.24 g/mol

Exact Mass: 270.09 g/mol

CAS#: 55520-64-4

Purity: ≥ 98 % (HPLC)

Form: solid

Color: white to off-white

Solubility: DMSO, methanol

Spectroscopic Properties: λ_max 288 nm, ε 8.5 L mmol^-1 cm^-1 (Methanol)

Applications:
RNA synthesis monitoring^[1]

Related products: 5-Ethynyl-uridine (5-EU), #CLK-N002

Selected References:
[1] Liu et al. (2019) A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjug. Chem. 30 (11):2958.