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Biotin Amine Protein Labeling

NHS Esters of Biotin enable Biotin labeling of both proteins (e.g. antibodies) via targeting of Lysine and any other primary amine-containing macromolecules. These biotinylated molecules are subsequently detected via fluoresecent or HRP-labeled streptavidin.

Binding of Biotin by avidin, streptavidin or NeutrAvidinTM is the strongest known biological interaction with a dissociation constant in the range of 10-15 M. The vitamin Biotin may be conjugated to many proteins without loss of their biological activity due to the small size of the Biotin molecule.