NHS Ester of Fluorescent Dyes enable one-step labeling & detection of both proteins (e.g. antibodies) via targeting of Lysine and any other primary amine-containing macromolecules.
In general, Thiol Labeling is more specific than Amine Labeling due to the relatively low abundance of cysteines compared to lysines. Since however, appearance of cysteines and lysines varies among different proteins, the amino acid sequence and tertiary structure of the protein of interest must be analyzed prior to deciding on the labeling strategy. The intended application of the fluorescent protein also determines the labeling strategy. Amine labeling is used for immunochemistry whereas Thiol labeling is preferred for investigating structure, function and protein interaction.
| Emission Color | Products | Abs max. [nm] | Em max. [nm] | Replacement for |
|---|---|---|---|---|
| blue-green | Atto 425 Protein Labeling Kit for primary amino groups | 436 | 484 | DEAC: 430 / 477 nm |
| green | Atto 488 Protein Labeling Kit for primary amino groups | 501 | 523 | Alexa Fluor 488: 495 / 519 nm Fluorescein (FITC): 495 / 520 nm FAM: 495 / 520 nm Oregon Green 514: 506 / 526 nm Rhodamine green: 503 / 528 nm Rhodamine 123: 507 / 529 nm |
| yellow- green | Atto 532 Protein Labeling Kit for primary amino groups | 532 | 553 | Alexa Fluor 532: 531 / 554 nm Bodipy 530/550: 534 / 554 nm |
| yellow- | Cy3 Protein Labeling Kit for primary amino groups | 550 | 570 | |
| yellow | Atto 550 Protein Labeling Kit for primary amino groups | 554 | 576 | Alexa Fluor 555: 555 / 565 nm Cy 3: 550 / 570 nm Alexa Fluor 546: 556 / 573 nm TAMRA: 546 / 576 nm Rhodamine Red: 560 / 580 nm Spectrum Orange: 559 / 588 nm |
| orange | Atto 590 Protein Labeling Kit for primary amino groups | 594 | 624 | Alexa Fluor 594: 590 / 617 nm Alexa Fluor 610: 612 / 628 nm |
| red | Atto 647N Protein Labeling Kit for primary amino groups | 644 | 669 | Alexa Fluor 647: 650 / 665 nm Cy 5: 643 / 667 nm |
| red | Cy5 Protein Labeling Kit for primary amino groups | 649 | 670 | |
| near-IR | Atto 655 Protein Labeling Kit for primary amino groups | 663 | 684 | Alexa Fluor 660: 664 / 691 nm Cy 5.5: 675 / 694 nm |
