5-Propargylamino-dUTP
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H18N3O14P3 (free acid)
Molecular Weight: 521.20 g/mol (free acid)
Exact Mass: 521.00 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 10.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Selected References:
Arango et al. (2018) Acetylation of Cytidine in mRNA Promotes Translation Efficiency. Cell 175 (7):1872.
Kuwahara et al. (2005) Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates. Nucleic Acids Symp. Ser. (Oxf). 49:275.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-998S | 10 μl (100 mM) | 130,80 € |
| NU-998L | 5 x 10 μl (100 mM) | 522,60 € |
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Scientific citations for Jena Bioscience nu-998
2 scientific citations and 0 citation images found by Bioz.
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Construction of a damage site-specific fluorescent biosensor for single-molecule detection of DNA damage.
Talanta, 2021-09-15 · PMID:34517666
Abstract: The 8-oxoguanine (8-oxoG) represents the most common DNA damage type, and it has been regarded as the oxidative stress biomarker, but the reported 8-oxoguanine assays are limited by poor specificity and low sensitivity. Herein, we demonstrate the construction of damage site-specific fluorescent biosensor for 8-oxoG assay by integrating single-molecule detection with hyperbranched signal amplification. In this assay, the 8-oxoG damages in DNA can generate free 3' OH with the assistance of formamidopyrimidine DNA glycosylase (Fpg) and polynucleotide kinase (PNK), which subsequently triggers the incorporation of abundant Cy5-labeled dUTPs via terminal deoxynucleotidyl transferase (TDT)-mediated site-specific hyperbranched nucleic acid amplification
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Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Genome, 2016-06-17 · PMID:26334496
.. ic ia l v er si on o f re co rd . ... 9 the DNA template or 50 nM
5-propargylamino-dUTP-5/6-TAMRA (TAMRA-dUTP;Jena Bioscience ) corresponding to the unmethylated T allele (Fig. 1, Reaction 2) at the target TpG site.. After an initial denaturation step for 3 min at 95°C, primer extension is carried ..
