γ-[(PEG₃-Amino)-imido]-ATP-Biotin

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₈H₄₈N₉O₁₇P₃S (free acid)

Molecular Weight: 907.72 g/mol (free acid)

Exact Mass: 907.21 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 259 nm, ε 15.3 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.0.

Selected References:
Arora et al. (2017) Unexpected biotinylation using ATP-c-Biotin-LC-PEO-amine as a kinase substrate. Biochem. Biophys. Res. Commun. 432:287.
Embogama et al. (2017) K-BILDS: A Kinase Substrate Discovery Tool. Chembiochem. 18 (1):136.
Ma et al. (2010) Highly sensitive detection of DNA phosphorylation by counting single nanoparticles. Anal. Bioanal. Chem. 397 (6):2279.
Seneviranthne et al. (2012) Kinase-Catalyzed Biotinylation. Curr. Protoc. Chem. Biol. 4 (1):83.
Seneviranthne et al. (2016) The generality of kinase-catalyzed biotinylation. Biorg. Med. Chem. 24 (1):12.
Green et al. (2007) Kinase-catalyzed biotinylation for phosphoprotein detection. J. Am. Chem. Soc. 129 (1):10.