α-[(6-Aminohexyl)imido]-AMP - Biotin, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C26H42N9O8PS (free acid)
Molecular Weight: 671.71 g/mol (free acid)
Exact Mass: 671.26 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 260 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
5'-labeling of RNA by in vitro transcription
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.0.
 Huang et al. (2008) Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription. Nat. Protoc. 3 (12):1848.