ddCTP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₉H₁₆N₃O₁₂P₃ (free acid)

Molecular Weight: 451.16 g/mol (free acid)

Exact Mass: 450.99 g/mol (free acid)

CAS#: 66004-77-1

Purity: ≥ 98 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 271 nm, ε 8.9 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
SNP: multiplexing by minisequencing^[1]
Multiplexed detection of alternatively spliced transcripts^[2]
Effect on reverse transcriptase of HIV-1^[3]
Molecular dynamic calculations of DNA-polymerase beta-ddCTP complex^[4]
Crystal structure with DNA- polymerase-I^[5]

BIOZ Product Citations:

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Selected References:
[1] Gronlund et al. (2011) Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap. Preparative Biochemistry and Biotechnology 41:166.
[2] Milani et al. (2006) Detection of alternatively spliced transcripts in leukemia cell lines by minisequencing on microarrays. Clinica Chemistry (Washington) 52:202.
[3] Anderson (2001) The molecular basis of inhibition and toxicity of modified cytosine analogues targetting HIV-1 reverse transcriptase. Antiviral Chemistry and Chemotherapy 12:13.
[4] Rittenhouse et al. (2003) Characterization of the active site of DNA polymerase beta by molecular dynamics and quantum chemical calculation. Proteins: Structure, Function and Genetics 53:667.
[5] Li et al. (2001) Crystal structures of ddATP-, ddTTP-, ddCTP-, and ddGTP-trapped ternary complex of Klentaq1: insights into nucleotide incorporation selectivity. Protein Science 10:1225.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.