Guanosine-5'-[(α,β)-methyleno]diphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H17N5O10P2 (free acid)
Molecular Weight: 441.23 g/mol (free acid)
Exact Mass: 441.05 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Color: white to off-white
Spectroscopic Properties: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Hydrolysis by tubulin
Influence on Z-DNA conformation
 Caplow et al. (1994) The free energy of hydrolysis of a microtubule-bound nucleotide triphosphate is near zero: All of free energy for hydrolysis is stored in microtubule lattice. J. Cell Biology 127:779.
 Ban et al. (1996) Crystal structure of the self-complementary 5'-purine start decamer d (GCGCGCGCGC) in the Z-DNA conformation. Biophysical J. 71:1215.
 Inoue et al. (1969) Low-field proton magnetic resonance spectra for seven dinucleotides ApCp, ApGp, ApUp, CpGp, GpCp, GpUp, and UpGp. Physiological Chemistry and Physics 1:77.
Martin-Galiano et al. (2011) Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor.J. Biol. Chem. 286 (22):19789.
Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta)methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.
Vulevic et al. (1997) Role of guanine nucleotides in the vinblastine-induced self-association of tubulin: effects of guanosine alpha,betamethylenetriphosphate and guanosine alpha,beta-methylenediphosphate. Biochemistry-US 36 (42):12828.
Hyman et al. (1995) Structural-changes accompanying GTP hydrolysis in microtubules - information from a slowly hydrolyzable analog guanylyl- (alpha,beta)-methylene-diphosphonate. J. Cell Biol. 128 (1-2):117.
Hyman et al. (1992) Role of GTP hydrolysis in microtubule dynamics - information from a slowly hydrolyzable analog, GMPCPP. Mol. Biol. Cell 3 (10):1155.