4-Thio-UMP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₉H₁₃N₂O₉PS (free acid)

Molecular Weight: 340.24 g/mol (free acid)

Exact Mass: 340.01 g/mol (free acid)

CAS#: 4145-46-4

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 331 nm, ε 16.3 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

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Selected References:
Sohn et al. (2005) Sequential multiple functions of the conserved sequence in sequence-specific termination by T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 102 (1):75.
Shen et al. (2001) Two site contact of elongating transcripts to phage T7 RNA polymerase at C-terminal regions. J. Biol. Chem. 276 (6):4080.
Powell et al. (1996) Elongation factor SII contacts the 3'-end of RNA in the RNA polymerase II elongation complex. J. Biol. Chem. 1271 (37):22301.
Sheng et al. (1993) Wheat germ and yeast RNA polymerase II: photoaffinity labeling by 4-thiouracil 5'-monophosphate positioned uniquely at the 3' end of an enzyme-bound [32P]-containing transcript. Biochemistry 32 (9):2248.
Khanna et al. (1991) Photoaffinity labelling of the pea chloroplast transcriptional complex by nascent RNA in vitro. Nucleic Acids Res. 19 (18):4849.
Kelly et al. (1990) Yeast RNA polymerase I. Derivatization of the 190 and 135 subunits by 4-thiouridine monophosphate positioned uniquely at the 3' terminus of an enzyme-bound 32P-containing transcript initiated by a triribonucleotide primer on synthetic single-stranded DNA. J. Biol. Chem. 265 (14):7787.
Shostak et al. (1990) Direct spectrophotometric assays for orotate phosphoribosyltransferase and orotidylate decarboxylase. Anal. Biochem. 191 (2):365.