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8-Azido-ATP

8-Azido-adenosine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-155S 500 μl (10 mM) 116,25 Add to Basket/Quote Add to Notepad
NU-155L 5 x 500 μl (10 mM) 407,36 Add to Basket/Quote Add to Notepad
Structural formula of 8-Azido-ATP (8-Azido-adenosine-5'-triphosphate, Sodium salt)
Structural formula of 8-Azido-ATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C10H15N8O13P3 (free acid)

Molecular Weight: 548.19 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 281 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP)[1]
Identification of protein ATP-binding sites[2]
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins

Selected References:
[1] Chen et al. (2004) Chain Termination and Inhibition of Saccharomyces cerevisiae Poly (A) Polymerase by C-8-modified ATP analogs. J. Biol. Chem. 279 (39):40405.
[2] Olcott et al. (1998) Localization and Characterization of Two Nucleotide-binding Sites on the Anaerobic Ribonucleotide Reductase from Bacteriophage T4. J. Biol. Chem. 273 (38):24853.