dITP - Solution

For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).

Description:
dITP, PCR-grade is supplied as ultrapure aqueous solution (pH
8.5) and suitable for all molecular biology applications including
PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C₁₀H₁₅N₄O₁₃P₃ (free acid)

Molecular Weight: 492.16 g/mol (free acid)

CAS#: 95648-77-4

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: λ_max 249 nm, ε 12.2 L mmol^-1 cm^-1 (Tris-HCl pH 7.0)

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.