hmdCTP, 5hmdCTP
5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-932S | 100 μl (100 mM) | 166,40 | Add to Basket/Quote Add to Notepad |
| NU-932L | 5 x 100 μl (100 mM) | 731,50 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H18N3O14P3 (free acid)
Molecular Weight: 497.18 g/mol (free acid)
Exact Mass: 497.00 g/mol (free acid)
CAS#: 7226-77-9
Purity: ≥ 95 % (HPLC), < 0.5% dCTP (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 275 nm, ε 7.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA by
- PCR with Taq polymerase in-house data, [1-6]
Description:
5-Hydroxymethylated DNA probes can be used as sequencing control [1-5] or for pull-down of 5-hmC binding proteins from cellular lysate[6].
BIOZ Product Citations:
Selected References:
[1] Booth et al. (2014) Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat. Chem. 6 (5):435.
[2] Booth et al. (2013) Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine. Nat. Protoc. 8 (10):1841.
[3] Yu et al. (2012) Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine. Nat. Protoc. 7 (12):2159.
[4] Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
[5] Szwagierczak et al. (2010) Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucl. Acids Res. 38(19):e181.
[6] Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.
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