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5-Hydroxymethyl-dCTP

hmdCTP, 5hmdCTP

5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
5-Hydroxymethyl-dCTP NU-932S 100 μl (100 mM) 125,00 Add to Basket/Quote Add to Notepad
5-Hydroxymethyl-dCTP NU-932L 5 x 100 μl (100 mM) 550,00 Add to Basket/Quote Add to Notepad
Structural formula of 5-Hydroxymethyl-dCTP (hmdCTP, 5hmdCTP, 5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt)
Structural formula of 5-Hydroxymethyl-dCTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C10H18N3O14P3 (free acid)

Molecular Weight: 497.18 g/mol (free acid)

CAS#: 7226-77-9

Purity: ≥ 95 % (HPLC), < 0.5% dCTP (HPLC)

Form: clear aqueous solution, pH 7.5 ±0.5

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 275 nm; ε 7.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Description:
5-Hydroxymethylated DNA probes can be used as sequencing control [1-5] or for pull-down of 5-hmC binding proteins from cellular lysate[6].

Applications:
Incorporation into DNA by
- PCR with Taq polymerase in-house data, [1-6]

Selected References:
[1] Booth et al. (2014) Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat. Chem. 6 (5):435.
[2] Booth et al. (2013) Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine. Nat. Protoc. 8 (10):1841.
[3] Yu et al. (2012) Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine. Nat. Protoc. 7 (12):2159.
[4] Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
[5] Szwagierczak et al. (2010) Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucl. Acids Res. 38(19):e181.
[6] Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.